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Immunohistochemical staining and comparative semi-quantitative analysis of <t>SERPINA1</t> and CFB in tubular and glomerular tissues from patients with ARKDs. ( A ) Immunohistochemical staining of SERPINA1 and CFB in tubular (top) and glomerular (bottom) tissue from patients with NC, MCN, MN, IgAN, and LN, respectively. ( B,C ) Comparative semi-quantitative analysis of immunohistochemical staining of SERPINA1 ( B ) and CFB ( C ) in tubular and glomerular tissue from patients with ARKDs. # Indicates a significant difference in the relative expression of SERPINA1 and CFB in each ARCKD group compared to the NC group, respectively ( P < 0.05). *Indicates a significant difference between tubular and glomerular tissue in each group ( P < 0.05).
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Immunohistochemical staining and comparative semi-quantitative analysis of <t>SERPINA1</t> and CFB in tubular and glomerular tissues from patients with ARKDs. ( A ) Immunohistochemical staining of SERPINA1 and CFB in tubular (top) and glomerular (bottom) tissue from patients with NC, MCN, MN, IgAN, and LN, respectively. ( B,C ) Comparative semi-quantitative analysis of immunohistochemical staining of SERPINA1 ( B ) and CFB ( C ) in tubular and glomerular tissue from patients with ARKDs. # Indicates a significant difference in the relative expression of SERPINA1 and CFB in each ARCKD group compared to the NC group, respectively ( P < 0.05). *Indicates a significant difference between tubular and glomerular tissue in each group ( P < 0.05).
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Immunohistochemical staining and comparative semi-quantitative analysis of SERPINA1 and CFB in tubular and glomerular tissues from patients with ARKDs. ( A ) Immunohistochemical staining of SERPINA1 and CFB in tubular (top) and glomerular (bottom) tissue from patients with NC, MCN, MN, IgAN, and LN, respectively. ( B,C ) Comparative semi-quantitative analysis of immunohistochemical staining of SERPINA1 ( B ) and CFB ( C ) in tubular and glomerular tissue from patients with ARKDs. # Indicates a significant difference in the relative expression of SERPINA1 and CFB in each ARCKD group compared to the NC group, respectively ( P < 0.05). *Indicates a significant difference between tubular and glomerular tissue in each group ( P < 0.05).

Journal: Scientific Reports

Article Title: Proteomic analysis of laser captured tubular tissues reveals complement activation and mitochondrial dysfunction in autoimmune related kidney diseases

doi: 10.1038/s41598-024-70209-9

Figure Lengend Snippet: Immunohistochemical staining and comparative semi-quantitative analysis of SERPINA1 and CFB in tubular and glomerular tissues from patients with ARKDs. ( A ) Immunohistochemical staining of SERPINA1 and CFB in tubular (top) and glomerular (bottom) tissue from patients with NC, MCN, MN, IgAN, and LN, respectively. ( B,C ) Comparative semi-quantitative analysis of immunohistochemical staining of SERPINA1 ( B ) and CFB ( C ) in tubular and glomerular tissue from patients with ARKDs. # Indicates a significant difference in the relative expression of SERPINA1 and CFB in each ARCKD group compared to the NC group, respectively ( P < 0.05). *Indicates a significant difference between tubular and glomerular tissue in each group ( P < 0.05).

Article Snippet: The sections were then incubated overnight at 4 °C with primary antibodies against Complement factor B (CFB) and SERPINA1 (rabbit anti-CFB, 1:100, and rabbit anti-SERPINA1, 1:100, both from ABclonal Technology, Wuhan, China).

Techniques: Immunohistochemical staining, Staining, Expressing